My question is why we use reverse-complement sequence instead of reversed sequence?How can I calculate colony forming unit (cfu) for bacteria? The primers are short DNA or RNA sequences which are complementary to the existing DNA strands. Notably, a pair of DNA primer, one for sense strand DNA called forward primer and one for antisense strand of DNA called reverse primer, is used for amplification of dsDNA. Each nucleotide contains a phosphate, a 5-carbon sugar molecule and a nitrogenous base.RNA only has one strand, but like DNA, is made up of nucleotides. The polymerase used in amplification is stable at a higher temperature and does not have the exonuclease activity. The term RNA primer is used loosely. RNA molecules on the other hand are more likely to be considered as primers as they are by nature single stranded and best suited to initiate replication. As you can see PCR amplification of DNA in vitro by providing a set of DNA primers. If I order primers for my PCR reaction they are DNA. )Primers are a very crucial ingredient in molecular genetic tools such as PCR or DNA sequencing. The evolutionary reason behind this is unclear, probably has something to do with the error rate of DNA pol or cell cycle control etc.DNA polymerase needs to a 3 prime hydroxyl group ( -OH ) in order to proceed adding new bases along the template.DNA Polymerase, requires a template and a primer (the -OH group on the THIRD carbon (not the second) of deoxyribose).
tRNA, like mRNA, is a free-roaming molecule that moves around the cytoplasm. The sugar in DNA is deoxyribose, which contains one less hydroxyl group than RNA’s ribose. It is a blueprint for all genetic information contained within an organismRNA converts the genetic information contained within DNA to a format used to build proteins, and then moves it to ribosomal protein factories. Due to its deoxyribose sugar, which contains one less oxygen-containing hydroxyl group, DNA is a more stable molecule than RNA, which is useful for a molecule which has the task of keeping genetic information safe.RNA, containing a ribose sugar, is more reactive than DNA and is not stable in alkaline conditions. Here, the RNA primers can not work efficiently because it is less stable than the DNA primers. Primers are required for DNA synthesis because no known DNA polymerase is able to initiate polynucleotide synthesis. Later it is replaced by DNA nucleotides via RNAse H (hybrid- removing the RNA -DNA hybrid) enzyme and ligase.Everyone has already answered that DNA primers are not available inside the cells which is the reason why at all priming is required for DNA synthesis. What structural difference(s) have made this possible? Whereas the polymerase used in the replication can not work at a higher temperature and have the 3’ to 5’ and 5’ to 3’ exonuclease activity. The RNA primer is a short stretch of nucleic acid made up of the single-stranded RNA molecule. For further information, including about cookie settings, please read our The process of DNA replication can't be initiated with out a primer. As the DNA ligase doesn't seal the nick between the RNA primer and the DNA, that nick can be used by DNAP to replace the RNA primer. Probe vs Primer: Probe is a small fragment of DNA/RNA used to detect the presence of the target sequence in a sample by molecular hybridization. Primer is a small stretch of DNA or RNA that serves as a starting point for DNA replication. How to convert this 12,000 xg to rpm .Why we use reverse-complement to convert nucleotide sequence to protein sequence instead of reversed sequence?when I get forward and reverse sequence for same PCR product, most converting software offering reverse-complement converting to the reverse sequence instead of reverse conversion to be able to translate nucleotide code to protein. For which experiments, I have to use these PCRs?Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this?Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this?
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dna primer vs rna primer